Western Blotting
Methods and Protocols
(Sprache: Englisch)
This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular...
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This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.
Inhaltsverzeichnis zu „Western Blotting “
1. The Early Days of BlottingEdwin Southern
2. Origins of Protein Blotting
Harry Towbin
3. Western Blotting: Remembrance of Things Past
W. Neal Burnette
4. Simian Virus 40 and Protein Transfer
Jaime Renart
5. Western Blotting: An Introduction
Biji T. Kurien and R. Hal Scofield
6. From Little Helpers to Automation
Harry Towbin
7. Spectrophotometric Methods to Determine Protein Concentration
J.P. Dean Goldring
8. Solubilization of Proteins: The Importance of Lysis Buffer Choice
Mandy Peach, Noelle Marsh, Ewa I. Miskiewicz, and Daniel J. MacPhee
9. Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis
Der-Yen Lee and Geen-Dong Chang
10. Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel
Ingrid Olsen and Harald G. Wiker
11. Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel
Biji T. Kurien and R. Hal Scofield
12. Slice Blotting
Graeme Lowe
13. Localizing Proteins by Tissue Printing
Rafael F. Pont-Lezica
14. Dot-Immunobinding Assay (Dot-Iba)
Sumi Surendran, Annamma Mathai, and Vishnampet Venkataraman Radhakrishnan
15. Analysis of Antibody Clonotype by Affinity Immunoblotting
Biji T. Kurien and R. Hal Scofield
16. Glycosaminoglycan Blotting and Detection after Electrophoresis Separation
Nicola Volpi and Francesca Maccari
17. A Well-Based Reverse-Phase Protein Array of Formalin-Fixed Paraffin-Embedded Tissue
Joon-Yong Chung and Stephen M. Hewitt
18. Quantitative Computerized Western Blotting in Detail
Dalit Talmi-Frank, Charles L. Jaffe, and Gad Baneth
19. Cationic Electrophoresis and Eastern Blotting
Engelbert Buxbaum
20. A Miniaturized Blotting System for Simultaneous Detecting of Different Autoantibodies
Ulrich Canzler, Holger Bartsch, Kai Großmann, Werner Lehmann, Karsten Conrad, Biji T. Kurien, Yaser Dorri, R. Hal Scofield, and Michael P. Bachmann
21. Proteomic Expressional Profiling of a Paraffin Embedded
... mehr
Tissue by Multiplex Tissue Immunoblotting
Joon-Yong Chung and Stephen M. Hewitt
22. Post-Staining Electroblotting for Efficient and Reliable Peptide Blotting
Der-Yen Lee and Geen-Dong Chang
23. Multistrip Western Blotting: A Tool for Comparative Quantitative Analysis of Multiple Proteins
Edita Aksamitiene, Jan B. Hoek, and Anatoly Kiyatkin
24. Western Blotting using PVDF Membranes and Its Downstream Applications
Setsuko Komatsu
25. Blotting from PhastGel to Membranes by Ultrasound
Joseph Kost and Aharon Azagury
26. Western Blotting of High and Low Molecular Weight Proteins Using Heat
Biji T. Kuien and R. Hal Scofield
27. Membrane Strip Affinity Purification of Autoantibodies
Biji T. Kurien
28. Strip Immunoblotting of Multiple Antigenic Peptides
Biji T. Kurien
29. Double-Blotting: A Solution to the Problem of Non-Specific Binding of Secondary Antibodies in Immunoblotting Procedures
Françoise Lasne
30. Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis
Marion L. Greaser and Chad M. Warren
31. Immunodetection of P-Selectin Using an Antibody to its C-Terminal Tag
Padmaja Mehta
32. Improvements and Variants of the Mutliple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening
Oscar Noya, Sandra Losada, Marilyan Toledo, Adriana Gauna, María Angelita Lorenzo, Henry Bermúdez, and Belkisyolé Alarcón de Noya
33. Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates
Harry Towbin
34. Immunoprecipitation: Western Blot for Proteins of Low Abundance
Edward P. Trieu and Ira N. Targoff
35. Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications
Ioannis N. Manoussopoulos and Mina Tsagris
36. Shift-Western Blotting: Separate Analysis of Protein and DNA from Protein-DNA Complexes
Matthias Harbers
37. Grid-Immunoblotting
Olga Yeliosof and R. Hal Scofield
38. Detection and Quantification of Protein-Protein Interactions by Far-Western Blotting
Joshua A. Jadwin, Bruce J. Mayer, and Kazuya Machida
39. Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay
Robert Sackstein and Robert Fuhlbrigge
40. Centrifuge Blotting
Jinny Paul
41. Blotting of Coomassie Blue Stained Proteins from PAGE Gels to Transparencies
Jinny Paul
42. B Cell ELISPOT: For the Identification of Antigen-Specific Antibody Secreting Cells
Hemangi B. Shah and Kristi A. Koelsch
43. T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells
Kerry M. Leehan and Kristi A. Koelsch
44. Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays
Alexander E. Kalyuzhny
45. SDS PAGE to Immunoblot in One Hour
Biji T. Kurien, Debashish Danda, Michael Bachmann, and R. Hal Scofield
46. Single Cell Western Blotting
Syed MS Quadri
47. Protein Detection by Simple Western(TM) Analysis
Valerie Harris
48. Western Blotting Using Microfluidics
Pothur R. Srinivas
49. Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis
Hisao Haniu, Daisuke Watanabe, Yusuke Kawashima, and Hiroyuki Matsumoto
50. Fingerprint Deposition of Nitrocellulose and Polyvinylidene Difluoride Membranes Using Alkaline Phosphatase
Biji T. Kurien, Debashish Danda, and R. Hal Scofield
51. Other Notable Protein Blotting Methods: A Brief Review
Biji T. Kurien and R. Hal Scofield
Joon-Yong Chung and Stephen M. Hewitt
22. Post-Staining Electroblotting for Efficient and Reliable Peptide Blotting
Der-Yen Lee and Geen-Dong Chang
23. Multistrip Western Blotting: A Tool for Comparative Quantitative Analysis of Multiple Proteins
Edita Aksamitiene, Jan B. Hoek, and Anatoly Kiyatkin
24. Western Blotting using PVDF Membranes and Its Downstream Applications
Setsuko Komatsu
25. Blotting from PhastGel to Membranes by Ultrasound
Joseph Kost and Aharon Azagury
26. Western Blotting of High and Low Molecular Weight Proteins Using Heat
Biji T. Kuien and R. Hal Scofield
27. Membrane Strip Affinity Purification of Autoantibodies
Biji T. Kurien
28. Strip Immunoblotting of Multiple Antigenic Peptides
Biji T. Kurien
29. Double-Blotting: A Solution to the Problem of Non-Specific Binding of Secondary Antibodies in Immunoblotting Procedures
Françoise Lasne
30. Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis
Marion L. Greaser and Chad M. Warren
31. Immunodetection of P-Selectin Using an Antibody to its C-Terminal Tag
Padmaja Mehta
32. Improvements and Variants of the Mutliple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening
Oscar Noya, Sandra Losada, Marilyan Toledo, Adriana Gauna, María Angelita Lorenzo, Henry Bermúdez, and Belkisyolé Alarcón de Noya
33. Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates
Harry Towbin
34. Immunoprecipitation: Western Blot for Proteins of Low Abundance
Edward P. Trieu and Ira N. Targoff
35. Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications
Ioannis N. Manoussopoulos and Mina Tsagris
36. Shift-Western Blotting: Separate Analysis of Protein and DNA from Protein-DNA Complexes
Matthias Harbers
37. Grid-Immunoblotting
Olga Yeliosof and R. Hal Scofield
38. Detection and Quantification of Protein-Protein Interactions by Far-Western Blotting
Joshua A. Jadwin, Bruce J. Mayer, and Kazuya Machida
39. Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay
Robert Sackstein and Robert Fuhlbrigge
40. Centrifuge Blotting
Jinny Paul
41. Blotting of Coomassie Blue Stained Proteins from PAGE Gels to Transparencies
Jinny Paul
42. B Cell ELISPOT: For the Identification of Antigen-Specific Antibody Secreting Cells
Hemangi B. Shah and Kristi A. Koelsch
43. T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells
Kerry M. Leehan and Kristi A. Koelsch
44. Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays
Alexander E. Kalyuzhny
45. SDS PAGE to Immunoblot in One Hour
Biji T. Kurien, Debashish Danda, Michael Bachmann, and R. Hal Scofield
46. Single Cell Western Blotting
Syed MS Quadri
47. Protein Detection by Simple Western(TM) Analysis
Valerie Harris
48. Western Blotting Using Microfluidics
Pothur R. Srinivas
49. Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis
Hisao Haniu, Daisuke Watanabe, Yusuke Kawashima, and Hiroyuki Matsumoto
50. Fingerprint Deposition of Nitrocellulose and Polyvinylidene Difluoride Membranes Using Alkaline Phosphatase
Biji T. Kurien, Debashish Danda, and R. Hal Scofield
51. Other Notable Protein Blotting Methods: A Brief Review
Biji T. Kurien and R. Hal Scofield
... weniger
Autoren-Porträt
Biji Kurien, PhD Associate Professor of Research Oklahoma Medical Research Foundation (OMRF) Arthritis & Clinical Immunology Research Program 825 NE 13th St. MS24 Oklahoma City, OK 73104 Tel: (405) 271-8000 ext 35204 Email: biji-kurien@ouhsc.edu Robert H. Scofield, MD Arthritis & Clinical Immunology Research Program, Oklahoma Medical Research Foundation 825 N.E. 13th Street. MS 38 Oklahoma City, OK 73104 Phone: (405) 271-7061 Fax: (405) 271-7063 E-mail: Hal-Scofield@omrf.org
Bibliographische Angaben
- 2015, 2015, XIV, 509 Seiten, 65 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Biji T. Kurien, R. Hal Scofield
- Verlag: Springer, Berlin
- ISBN-10: 1493926934
- ISBN-13: 9781493926930
- Erscheinungsdatum: 05.06.2015
Sprache:
Englisch
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